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Bioss
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Servicebio Inc
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Boster Bio
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Cell Signaling Technology Inc
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Servicebio Inc
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fluidigm
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fluidigm
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Fisher Scientific
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Proteintech
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Journal: Research
Article Title: Mapping Immune-Inflammatory Niches on Zirconia Bone Implants: Single-Cell Transcriptomic Profiling
doi: 10.34133/research.1162
Figure Lengend Snippet: Intercellular cross talk and functional enrichment in the bone-marrow microenvironment following titanium (Ti) implantation. (A and B) Cells extracted from the Ti implant (A) and their proportions compared with those in the sham group (B). (C) Fuzzy C -means clustering of the differentially expressed genes (DEGs) and their functional annotation via Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses. BP, Biological Process; CC, Cellular Component; MF, Molecular Function. (D and E) Outgoing–incoming cross talk analysis among the bone-marrow cells and their subpopulations. (F) Outgoing–incoming signaling patterns and associated interaction strengths. (G) Analysis of collagen-related signaling networks and intercellular interaction strengths. (H) Interaction-strength analysis of candidate ligand–receptor pairs in bone-marrow fibroblasts. (I) KEGG and GO (BP, CC, and MF) enrichment analyses of the DEGs in bone-marrow fibroblasts. (J and K) Multiplex fluorescence colocalization and costaining analysis of candidate ligand–receptor pairs. Scale bars: 100 and 20 μm. COL1A1, collagen type I alpha 1 chain; SDC1, syndecan 1; ATP, adenosine triphosphate; DAPI, 4′,6-diamidino-2-phenylindole.
Article Snippet: The following antibodies were applied sequentially: CD44 (Bioss, bsm-54767R, 1:300, China), CD68 (Proteintech, 66231-2-Ig, 1:500, China), CD206 (Bioss, bsm-55604R, 1:300, China), NOS2 (Bioss, bs-0162R, 1:300, China), COL6A2 (Bioss, bs-13963R, 1:300, China), S100A4 (Bioss, bs-3759R, 1:300, China), COL1A1 (Bioss, bs-10423R, 1:300, China), and
Techniques: Functional Assay, Multiplex Assay, Fluorescence
Journal: Research
Article Title: Mapping Immune-Inflammatory Niches on Zirconia Bone Implants: Single-Cell Transcriptomic Profiling
doi: 10.34133/research.1162
Figure Lengend Snippet: Validation of the specific ligand–receptor pairs between the implants and bone-marrow cells. (A) Schematic diagram of the rat model of intramedullary femoral implantation and bulk RNA sequencing workflow. (B) Violin plots showing the expression levels of Col6a2 , Cd44 , Col1a1 , and Sdc1 . (C and D) Hierarchical clustering heatmaps of pairwise DEGs among the sham, Ti, and ZrO 2 groups. (E to G) Identification of the gene modules associated with Ti and ZrO 2 via weighted gene coexpression network analysis. (H) Overlapping genes between module-related genes and DEGs in each group. (I) Contributions of Ti and ZrO 2 -associated feature genes to predictive outcomes, assessed using least absolute shrinkage and selection operator (LASSO) regression analysis. (J and K) Temporal expression patterns and correlations of Col6a2 , Cd44 , Col1a1 , and Sdc1 . Statistical significance was assessed using the t test.
Article Snippet: The following antibodies were applied sequentially: CD44 (Bioss, bsm-54767R, 1:300, China), CD68 (Proteintech, 66231-2-Ig, 1:500, China), CD206 (Bioss, bsm-55604R, 1:300, China), NOS2 (Bioss, bs-0162R, 1:300, China), COL6A2 (Bioss, bs-13963R, 1:300, China), S100A4 (Bioss, bs-3759R, 1:300, China), COL1A1 (Bioss, bs-10423R, 1:300, China), and
Techniques: Biomarker Discovery, RNA Sequencing, Expressing, Selection